pH responsive ATP carriers to drive kinesin movement.
نویسندگان
چکیده
Multilayer film coated CaCO3 microspheres were employed as pH responsive ATP carriers to drive kinesin movement. The production of oxygen scavengers in a kinesin-microtubule system induces the decomposition of ATP-loaded CaCO3 microspheres and then leads to the release of ATP.
منابع مشابه
Allele-specific activators and inhibitors for kinesin.
Members of the kinesin superfamily are force-generating ATPases that drive movement and influence cytoskeleton organization in cells. Often, more than one kinesin is implicated in a cellular process, and many kinesins are proposed to have overlapping functions. By using conventional kinesin as a model system, we have developed an approach to activate or inhibit a specific kinesin allele in the ...
متن کاملKinesin-8s hang on by a tail
Accurate segregation of genetic material into two daughter cells is essential for organism reproduction, development, and survival. The cell assembles a macromolecular structure called the mitotic spindle, which is composed of dynamic microtubules (MTs) and many associated proteins that assemble the spindle and drive the segregation of the chromosomes. Members of the kinesin superfamily of MT a...
متن کاملStimuli-responsive nucleic acid-functionalized metal–organic framework nanoparticles using pH- and metal-ion-dependent DNAzymes as locks† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c7sc01765k Click here for additional data file.
A versatile approach to modify metal–organic framework nanoparticles (NMOFs) with nucleic acid tethers, using the “click chemistry” method is introduced. The nucleic acid-functionalized NMOFs are used to prepare stimuli-responsive carriers of loads (fluorescence probes or anti-cancer drugs). Two different stimuli-responsive nucleic acid-based NMOFs are presented. One system involves the prepara...
متن کاملCharacterization of the microtubule movement produced by sea urchin egg kinesin.
We have used an in vitro assay to characterize some of the motile properties of sea urchin egg kinesin. Egg kinesin is purified via 5'-adenylyl imidodiphosphate-induced binding to taxol-assembled microtubules, extraction from the microtubules in ATP, and gel filtration chromatography (Scholey, J. M., Porter, M. E., Grissom, P. M., and McIntosh, J. R. (1985) Nature 318, 483-486). This partially ...
متن کاملControlling Kinesin by Reversible Disulfide Cross-Linking
Conventional kinesin, a dimeric molecular motor, uses ATP-dependent conformational changes to move unidirectionally along a row of tubulin subunits on a microtubule. Two models have been advanced for the major structural change underlying kinesin motility: the first involves an unzippering/zippering of a small peptide (neck linker) from the motor catalytic core and the second proposes an unwind...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Chemical communications
دوره 51 65 شماره
صفحات -
تاریخ انتشار 2015